Use of biuret reagent to test

Biuret test principle pdf

Application of an improved biuret method to the determination of total protein in urine and cerebrospinal fluid without concentration step by use of Hitachi auto-analyzer. Add ml of the test solution, egg albumin and deionized water in the respective test tubes. Secondary wavelength was nm. Biuret test is a general test for compounds proteins and peptides having two or more peptide CO-NH bonds. A cell-free cerebrospinal fluid sample produced by centrifugation can be inserted in an auto-analyzer for protein measurement directly. Add precipitant Na 2 WO 4 to urine samples. It is believed that the color is due to the formation of a copper co-ordinated complex or chelate complex. What is the procedure for the Biuret Test for protein using sodium hydroxide and copper sulphate solutions? The test results were calculated against a one-point standard.

J Clin Lab Anal. The color change from blue to violet indicates the presence of proteins.

biuret test principle

The Biuret test for proteins may also be extended to quantitatively measure the concentration of total protein using spectrometric methods. Shake well and allow the mixtures to stand for 5 minutes.

biuret test reaction equation

This biuret colorimetric method was relatively simple, fast, and accurate for the determination of protein in urine and cerebrospinal fluid, with a wide linearity extending from 0.

The peptide bonds in Biuret give a positive result for the test hence the reagent is named so. The chelate complex absorbs light at nm and hence appears violet.

biuret test for urea

The quantity of food crushed and water used depends on the number of tests to be conducted. Cupric ions or Cu II ions form a violet-colored chelate complex with unshared electron pairs of peptide nitrogen and oxygen of water.

October 16, Author Biochemistry 0 Biuret is a compound formed by heating urea at It is the result of the condensation of 2 molecules of urea.

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Biuret test: Principle, Requirements, Procedure and Result Interpretation