A lab study conducted to determine the concentration of vitamin c in various samples using titration

If you run out of stock or spill this solution accidentally you will need to repeat part A on the new solution.

Determination of ascorbic acid in vitamin c tablets lab report

This applies to all three parts of the experiment. Calculate the molarity of this sample. As you become proficient in performing titrations you will get a "feeling" for how much to open the stopcock to deliver just one drop of titrant. Wash the filter with a few milliliters of distilled water. Many fruits and vegetables contain vitamin C, but cooking destroys the vitamin, so raw citrus fruits and their juices are the main source of ascorbic acid for most people. Fresh Fruit Juice - Strain the juice through a coffee filter or cheesecloth to remove pulp and seeds, since they could get stuck in the glassware. Label the flask as your vitamin C standard solution. Add distilled water to make ml of solution in a volumetric flask. Standardizing Solutions Add A black or white card held up behind the buret helps with making this reading. Repeat any trials that seem to differ significantly from your average. Results showed that ascorbic acid concentrations after being exposed to increased heat for 5 minutes were lowest.

By having one beaker of stock you ensure that all your trials come from the same solution. It is not necessary that you weigh out the exact mass you calculated, so long as you record the actual mass of ascorbic acid added in each trial for your final calculations.

The purpose of this experiment is to test the levels of vitamin C in oranges of varying sizes.

A lab study conducted to determine the concentration of vitamin c in various samples using titration

Weigh out approximately this amount of ascorbic acid directly into a mL Erlenmeyer flask. What is the function of each? Packaged Fruit Juice - This also may require straining. Titrate until you have at least three measurements that agree within 0. Your results should be accurate to at least three significant figures. Rinsing: Always rinse a buret including the tip before filling it with a new solution. Calculate milligrams of ascorbic acid per gram of sample and using the average mass of a tablet, determine the number of milligrams of Vitamin C contained in each tablet. Liquid samples If you are using a pulpy juice, strain out the majority of the pulp using a cloth or filter. Record the initial volume. Be sure to swirl the solution to rinse all surfaces.

Removing Air Bubbles: Often air bubbles will be trapped in the tip of a newly filled buret. The macromolecules tested for were carbohydrates, proteins, and vitamin C. Note that not all of the tablet may dissolve as commercial vitamin pills often use calcium carbonate which is insoluble in water as a solid binder.

Determination of ascorbic acid in vitamin c tablets calculations

Work carefully: your grade for this experiment depends on the accuracy and precision of each of your final results. Vitamin C Standard Solution Dissolve 0. Rinsing: Always rinse a buret including the tip before filling it with a new solution. Dissolve the sample in about mL of deionized water and swirl well. Perform two more trials. Helmenstine holds a Ph. If you use a funnel to fill the burets be sure it is cleaned and rinsed in the same way as the burets and removed from the buret before you make any readings to avoid dripping from the funnel into the buret. We've listed examples of quantities, but they aren't important. Removing Air Bubbles: Often air bubbles will be trapped in the tip of a newly filled buret. Calculate the milligrams of ascorbic acid per milliliter of juice.

Titrate the solution until the endpoint is reached. The starting volumes in each of the burets should be between 0.

vitamin c determination methods

Fresh Fruit Juice - Strain the juice through a coffee filter or cheesecloth to remove pulp and seeds, since they could get stuck in the glassware.

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Vitamin C Analysis (Experiment)